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KMID : 0366319910110010011
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Biochemistry and Molecular Biology News
1991 Volume.11 No. 1 p.11 ~ p.13
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Abstract
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A database of 118 amino acid sequence of pyridoxal 5´-phosphate (PLP)-dependent enzyme was compiled. The aminotransferases with a total of 30 amino acid sequence of 7 different enzymes constitute the largest group among them. These 7 aminotransferases could be classified in to three families of the basis of sequence similarity. Aspartate aminotransferase family (family I) comprises aspartate, aromatic amino acid, and tyrosine am inotransferases; co-amino acid aminotransferase family (family II) w-amino acid: pyruvate aminotransferase and ornithine S-aminotransferase; Damino aminotransferase family (family III) D-amino acid aminotransferase and L-branched-chain amino acid aminotransferase. Comparison of the amino acid sequence with those of family I from other organisms revealed very low overall similarities (13%). Several amino acid residues conserved in all the compared sequence include those that have been reported to participate in binding of the coenzyme in three-dimensional structures of the vertebrate and E. coli enzymes. In family I and II the sequence identity after alignment was 35% and 31%, respectively.
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